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Peptidsynthese Labor Genosphere Biotechnologies - Stuttgart

Identification of Phosphorylation Sites Regulating sst3 Somatostatin Receptor Trafficking.

Lehmann, A., Kliewer, A., Günther, T., Nagel, F. and Schulz, S., 2016

Molecular Endocrinology, 30(6), 645-659.


Abstract

The human somatostatin receptor 3 (sst3) is expressed in about 50% of all neuroendocrine tumors and hence a promising target for multireceptor somatostatin analogs. The sst3 receptor is unique among ssts in that it exhibits a very long intracellular C-terminal tail containing a huge number of potential phosphate acceptor sites. Consequently, our knowledge about the functional role of the C-terminal tail in sst3 receptor regulation is very limited. Here, we have generated a series of phosphorylation-deficient mutants that enabled us to determine crucial sites for its agonist-induced β-arrestin mobilization, internalization, and down-regulation. Based on this information, we generated phosphosite-specific antibodies for C-terminal Ser(337)/Thr(341), Thr(348), and Ser(361) that enabled us to investigate the temporal patterns of sst3 phosphorylation and dephosphorylation. We found that the endogenous ligand somatostatin induced a rapid and robust phosphorylation that was completely blocked by the sst3 antagonist NVP-ACQ090. The stable somatostatin analogs pasireotide and octreotide promoted clearly less phosphorylation compared with somatostatin. We also show that sst3 phosphorylation occurred within seconds to minutes, whereas dephosphorylation of the sst3 receptor occurred at a considerable slower rate. In addition, we also identified G protein-coupled receptor kinases 2 and 3 and protein phosphatase 1α and 1β as key regulators of sst3 phosphorylation and dephosphorylation, respectively. Thus, we here define the C-terminal phosphorylation motif of the human sst3 receptor that regulates its agonist-promoted phosphorylation, β-arrestin recruitment, and internalization of this clinically relevant receptor.


EMMPRIN regulates β1 integrin-mediated adhesion through Kindlin-3 in human melanoma cells.

Delyon J et al. (2015) Experimental dermatology, 24(6), pp. 443-448


TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.

Blackford A N et al. (2015) Mol cell 57, 1133-1141




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