RGD Peptide in der Oberflächenbeschichtung

Allgemeine Anwendungsbeispiele.

Arg-Gly-Asp-Ser (RGDS peptide) is an integrin binding sequence that inhibits integrin receptor function; decreases systemic inflammation via inhibition of collagen-triggered activation of leukocytes and attenuates expression of inflammatory cytokines, iNOS and MMP-9.

[1]. Moon C, et al. Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways. Respir Res. 2009 Mar 9;10:18.

[2]. Yin X, et al. Synthetic RGDS peptide attenuated lipopolysaccharide/D-galactosamine-induced fulminant hepatic failure in mice. J Gastroenterol Hepatol. 2014 Jun;29(6):1308-15.

The surface (bio)chemistry of smooth and rough (SLA; particle-blasted and acid-etched) titanium surfaces was modified using the non-fouling polycationic co-polymer poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) known to impart protein resistance to negatively charged surfaces (e.g., titanium dioxide). Cell-interactive peptides with the RGD, KRSR and FHRRIKA motives were covalently grafted to a fraction of the PEG-chains

The consensus heparin-binding peptides sequences KRSR and FHRIKKA were also immobilized via PLL-g-PEG chemistry to SLA titanium surfaces and tested for their ability to support rat calvarial osteoblast and human gingival fibroblast proliferation after 7 days in culture. Cell-binding peptide sequence RGD in combination with KRSR or FHRRIKA was used to examine a possible enhanced effect on rat calvarial osteoblast attachment and proliferation.

Preliminary results have also shown that growth factors or drug delivery carriers can also be attached to PLL-g-PEG, thus demonstrating that in principle it should be possible to provide cells on the titanium surface with suitable attachment factors, growth factors, and other components to enhance osseointegration

Genosphere Biotechnologies - Peptide und Antikörper für die Forschung. Custom Peptides & Antibodies.