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Fast DNA Bisulfite Conversion Kit with Magnetic Beads (C --> U)

Katalog Nummer : 25-6010 DNA

5 Kit x 18 rxn
zum Vorteilspreis von nur
545 € zzgl. gesetzliche MwSt.

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DNA Bisulfite Conversion Kit

DNA Bisulfite Conversion Kit with Magnetic Beads provides a simple, rapid, efficient, high-throughput method for the conversion of unmethylated cytosines (C) of DNA into uracils by sodium bisulfite, while 5-methylcytosines (5mC) remain unchanged. The converted DNA can be purified with magnetic beads provided in the kit, and used for PCR amplification or high-throughput sequencing to detect and analyze DNA methylations.
This kit is ideal for high-throughput 96-well operations and is also suitable for routine single-tube operations. To facilitate 96-well operations, a 96-well deep well plate is supplied with this kit.

DNA methylation is an important epigenetic mechanism involving the transfer of a methyl group onto C5 position of the cytosine in DNA to form 5-methylcytosine (5mC) [1]. In eukaryotic genomes, the most common DNA modification is 5mC and CpG dinucleotides are the major methylation sites [2].
DNA methylation plays a significant role in regulating gene expression, maintaining chromatin structure, genomic imprinting, X chromosome inactivation, embryonic development, and other biological processes [3]. It has a profound impact on the onset and progression of aging and diseases [4]. It has been demonstrated that DNA methylation is closely related to various diseases, such as tumors, mental disorders, metabolic diseases, and autoimmune diseases [5]. DNA methylation can serve as a molecular marker or target to assist the precise diagnosis and treatment of diseases [4].
This product enables high cytosine conversion rates of over >99% with good reproducibility, making it the gold standard for DNA methylation analysis. When DNA is treated with sodium bisulfite, unmethylated cytosine undergoes sulphonation, deamination, desalting, and desulphonation, ultimately converting into uracil, while methylated cytosine remains in its original form.

Bisulfite conversion of cytosine (C) in DNA into uracil.
This method combines heat denaturation and bisulphite conversion steps for DNA treatment, followed by purification with magnetic beads. The desalination and desulphonation are completed during the DNA purification, making this method simpler and more convenient to use. It is suitable for automated high-throughput platforms and the whole process can be completed within 3 hours.

The workflow of DNA Bisulfite Conversion Kit with Magnetic Beads
The conversion products can be used for various downstream applications, such as methylation-specific PCR (MSP), bisulfite sequencing PCR (BSP), high-throughput sequencing, and restriction endonuclease analysis.
This kit enables high conversion rate and good DNA recovery. The applicable DNA amount is 10ng-2μg, with 0.5-2μg being more effective.

Katalog Nummer: 25-6010 DNA
DESCRIPTION [107 KB]
Germany:
Sansunbiologics

CONTACT US
Sansunbiologics
Eurotec Ring 15
47445 Moers
CONTACT：Hongyan Shi
EMAIL：info@sansunbiologics.com

Plant Tissue Direct PCR Kit

T-P-004 Plant Tissue Direct PCR Kit

100 rxns × 20 μL

Product Description

The kit is designed for direct PCR of different types of plant tissues. Simply treated plant tissues can be directly used as templates for PCR, without genome extraction. The product is compatible with common plant samples and polysaccharide polyphenol plant samples.

The 2×Plant Direct PCR Master Mix provided by this kit is specially optimized for direct amplification of plant tissue samples, which greatly improves the amplification efficiency and anti-inhibitor effect. In addition, the reagent contains all components except templates and primers, and the addition of dyes, the amplification products can be directly electrophoresis, which greatly simplifies the operation process. The simplicity and strong compatibility of this product can be used in molecular biology experiments such as rapid identification of transgenic plants and plant genotyping.

Storage and Handling Conditions

2× Plant Direct PCR Master Mix should be shipped with wet ice, and stored at -20°C; Other reagents are shipped with wet ice, and stored at 2~8°C; valid for up to 12 months.

Further similar Kits : Blood direct PCR Kit [385 KB]

DNA Extraction from Plants

Genomic DNA can be extracted from plant tissue samples rich in polysaccharides and polyphenols weighing between 20 and 300 mg, without the use of organic reagents such as phenol and chloroform, as well as the need for multiple centrifugation steps.

Product Name
MagBind Plant Genomic DNA Extraction Kit For Polysaccharides & Polyphenolics

Description
Through the specially optimized lysis buffer system, combined with the characteristics of specific binding of superparamagnetic beads to DNA, this kit can extract genomic DNA safely, quickly and efficiently from 20-300 mg (the initial amount of plant tissue varies depending on the sample) plant tissue samples (leaves, seeds, fruits, etc) rich in polysaccharides and polyphenols. The specially optimized lysis buffer system can remove impurities such as polysaccharides, polyphenols, proteins and cell fragments from plant samples. This kit does not require phenol, chloroform and other organic reagents, and does not require multi-step centrifugation. The extracted genomic DNA can be used in subsequent PCR reaction, enzyme digestion reaction, Southern hybridization, RAPD, AFLP, RFLP and many conventional molecular biology experiments.

Storage and Handling Conditions
RNase A is shipped with wet ice and stored at -20°C; The remaining reagents are shipped and stored at room temperature; The expiration date is 12 months.

Katalog Nummer: 25-5010 EXT
info@sansunbiologics.com

Animal Tissue Direct PCR Kit

T-P-007 Animal Tissue Direcht PCR Kit

Packaging: 100 T and 500 T are available

Product Description

The product is specially designed for amplification directly from animal tissues. Applicable samples include fresh or frozen mammalian viscera, nails, hair, zebrafish, drosophila, cultured cells, etc. The unique Animal Tissue Lysis Buffer A combine with Animal Tissue Lysis Buffer B to quickly release genomic DNA in micro tissues, without the need of genome extraction and purification, which is easy to operate, reduces the loss of samples, and greatly shortens the time-consuming experiments. The lysis product can be stored at -20℃ for a long time.

2× Animal Tissue Direct PCR Mix with Dye contains all components required for PCR except templates and primers. The DNA Polymerase is capable of amplifying rapidly and resist inhibitors after modification. The PCR Mix also contains a variety of reaction enhancers, which can further improve the amplification efficiency and fidelity, also amplify target genes up to 3 kb.



Storage and Shipping Conditions

Ship with wet ice; Animal Tissue Lysis Buffer A, stored at 4℃; Other components shall be stored at -20℃; Valid for 12 months.

Further similar Kits : Blood direct PCR Kit [385 KB]

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Genome-Editing Mutation Detection Kit

Genome-Editing Mutation Detection Kit provides a simple, reliable, and rapid method for detecting the mutations on genomic DNA extracted from cells or tissues subjected to CRISPR/Cas9 or other gene-editing processes. It employs the High-Fidelity DNA polymerase for amplifying the target DNA fragments, and the T7 Endonuclease I (T7E1) for recognizing and cleaving non-perfectly matched DNA (heteroduplex DNA).

Genome-Editing Mutation Detection Kit contains all reagents for DNA extraction, PCR amplification with high fidelity, and T7E1 enzyme digestion. It also contains positive control template DNA and its corresponding primers for troubleshooting. The DNA extracts and PCR products can be added directly into the subsequent reactions without extra purification, enabling the kit convenient to use.

Working mechanism of Genome-Editing Mutation Detection Kit: Using the genomic DNA extracted from cells or tissues subjected to gene-editing processes as template, target DNA region that possibly contains mutations is amplified by High-Fidelity DNA polymerase. The PCR products are then denatured and annealed, followed by digestion with T7E1 enzyme which cleaves heteroduplex DNA only. If mutations exist in target DNA, heteroduplex will be formed between unmutated DNA and mutated DNA, or between mutated DNA with different mutations. The mutated DNA is then cleaved by T7E1 into smaller fragments. Based on the concentrations of cleaved and uncleaved fragments, the efficiency of genome editing can be estimated.

Control Template provided in this kit contains both unmutated and mutated gene fragments. Amplicons of unmutated fragments are 525bp in length, and those of mutated fragments with 7nt deletion mutation are 518bp in length. The mutated DNA strand in the Heteroduplex DNA formed between the unmutated and the mutated DNA fragments is cleaved by T7E1 into two DNA fragments, 288bp and 230bp in length, respectively. Meanwhile, the homoduplex of either unmutated or mutated DNA could not be cleaved by T7E1. Therefore, after T7E1 digestion, positive control samples should contain DNA fragments of four different sizes (525bp, 518bp, 230bp, and 288bp). Usually, the 525bp and 518bp bands are nearly together when separated on agarose gels.


Katalog Nummer: 25-6050 DNA

Germany:
Sansunbiologics

CONTACT US
Sansunbiologics
Eurotec Ring 15
47445 Moers
CONTACT：Hongyan Shi
EMAIL：info@sansunbiologics.com

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