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Labels, fluorophore, FRET

7-Amino-4-methylcoumarin, C-terminal, [amc]  

Benzyloxycarbonyl, [cbz]  

Biotin, N-terminal [biot]  

Biotin-Lys, C-terminal, internal, [K-biot]  

Dabcyl, N-terminal, [dabcyl]  

Dabcyl-Lys, C-terminal, internal, [K-dabcyl]  

Dansyl, N-terminal [dans]  

Dansyl-Ethylenediamine, C-terminal, [ed-dansyl]  

Dansyl-Lys, C-terminal, internal, [K-dansyl]  

Dinitrophenyl-Diaminopropionic acid, N-terminal, [dap-dnp]  

Dinitrophenyl-Ethylenediamine, C-terminal, [ed-dnp]  

Dinitrophenyl-Lys, N-terminal, internal, [K-dnp]  

EDANS, C-terminal [edans]  

EDANS-Glu, N-terminal, internal, [E-edans]  

Fluorescein, N-terminal, [fl]  

Fluorescein-Lys, C-terminal, internal, [K-fl]  

Methoxycoumarin acetic acid, N-terminal, [mca]  

Methoxycoumarin acetic acid-Lys, C-terminal, internal, [K-mca]  

p-Nitroaniline, C-terminal, [pNA]  

Tetramethylrhodamine, N-terminal, [tmr]  

Tetramethylrhodamine-Lys, C-terminal, internal, [K-tmr]


N-terminal fatty acids conjugation

Myristic acid, [myr]  

Palmitic acid, [palm]  

Stearic acid, [stea]  

Oleic acid, [ole]  

Linoleic acid, [lin]  

Protein carrier conjugation



Disulfide bridge (s)  

Head to tail lactam  

Internal lactam, lactone  


Spacers, linkers

C2, C4, C6, C8, C10, C12 methylene spacers  

Polyethylene glycol (PEG, PEG2, PEG3...)  

Laurent Fourmi hat noch mehr Modifikationen im Angebot ! Voilá

 ….  we tested the proteolytic activity of recombinant shewasin D using five different fluorogenic peptides typically used as substrates of APs with different origins. None of the three substrates: a renin substrate Arg-Glu-(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys-(DABCYL)-Arg, a BACE-1 substrate [MCA-Lys]-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-[Lys-DNP], or [MCA-Lys]-Ala-Leu-Ile-Pro-Ser-Tyr-Lys-Trp-Ser-[Lys-DNP] was cleaved under the conditions applied. The lack of shewasin D activity towards BACE-1 substrate suggests different substrate preferences, despite the sequence similarity and relatively close phylogenetic relationship of this bacterial pepsin-like AP with mammalian BACE-1 and BACE-2 Interestingly, shewasin D also failed to cleave the fluorogenic peptide (MCA)Lys-Leu-His-Pro-Glu-Val-Leu-Phe-Val-Leu-Glu-Lys(DNP), which was effectively hydrolyzed by shewasin A. The only fluorogenic peptide cleaved by recombinant shewasin D was (MCA)Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys(DNP), a substrate widely used to assess proteolytic activity of A1 APs. This enzyme displayed maximum activity towards this substrate at pH values between pH 3.5 and 4.0, with a drastic decrease in activity of 50% and 70% at pH values 3.0 and 5.0, respectively  ….   

Synthesis of EDANS -  Dabcyl [Donor -  Quencher] Pair Peptides