Peptidmodifikationen
FRET Substrates
| Fluorophore | Please choose your individual amino acid sequence (linear or cyclic) | Quencher |
|---|---|---|
| FAM | Your individual amino acid sequence (linear or cyclic) | Dabcyl |
| Trp | Your individual amino acid sequence (linear or cyclic) | Dnp |
| Abz | Your individual amino acid sequence (linear or cyclic) | Dnp |
| Mca | Your individual amino acid sequence (linear or cyclic) | Dnp |
| EDANS | Your individual amino acid sequence (linear or cyclic) Make your choice. Send us your request. | Dabcyl |
FITC-LC-Peptid anstelle von FITC-Peptid
Wir empfehlen bei der Auswahl von Fluorescein (FITC) als N-terminalem Fluoreszenzlabel dieses mit einem "long chain (C6 spacer / Ahx [ 6-amino caproic acid / 6-amino hexanoic acid]" von der nachfolgenden Peptidsequenz zu trennen.
Das Label "FITC" zeigt, im Gegensatz zum Label "FAM" die Tendenz, sich während der gewünschten Markierungsreaktion der Peptide gemeinsam mit der N-terminalen Aminosäure abzuspalten. Vergleiche hierzu : Edman Degradation.
Der bei der Peptidkonjugation mit dem FITC-Farbstoff unerwünschte Degradationsvorgang kann durch einfügen eines LC Spacers zwischen FITC und Peptidsequenz weitesgehend vermieden werden.
Non-standard residues
Aminoisobutyric acid, [aib]
Azido-Lysin, [K-n3] **)
Beta aspartic acid, [beta D]
Beta homo amino acids, [beta homo-aa]
Beta-alanine, [beta A]
Citrullin, [cit]
4-Carboxyglutaminsäure, Gla, (HOOC)2CH-CH2-CH(NH2)-COOH
Cyclohexylalanine, [cha]
D amino-acids, [d-aa]
Dihydroxyphenylalanine, [dopa]
Epsilon lysine, [epsilon K]
Fluoro-, chloro-, bromo phenylalanine, [x-F]
Fluoro-, chloro-, bromo tyrosine, [x-Y]
Gamma glutamic acid [gamma E]
Hydroxyproline, [hyp]
K-Biotin , Lys-N-epsilon-Biotin
Methyl-, acetyl-lysine [meK], [acK] N-methyl amino-acids, [N-Me-aa]
Norleucine, [nle]
Pencillinamine, [pen]
Phosphorylation [pS], [pT], [pY]
**) Azides of amino acids can be labeled with terminal Alkyne or strained Alkyne (e.g. DBCO)-tagged reporter molecules via a Cu(I)-catalyzed Alkyne-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.
Some examples for possible applications:
Furin substrate :L-Pyr-Arg-Thr-Lys-Arg-AMC (AMC = 7-Amino-4-methylcoumarin).
or
Cathepsin K substrate (fluorogenic)
Sequence: Z-Leu-Arg-AMC . HCl
[Z=Cbz=Benzyloxycarbonyl; AMC=7-amino-4-methylcoumarin]
Z-LR-AMC is also cleaved by malaria parasite cysteine proteases falcipain-1, -2, -3
Heavy isotope (13C, 15N) labeled amino-acids
13C15N-K
13C15N-R
13C15N-A
13C15N-I
13C15N-L
13C15N-F
13C15N-P
13C15N-V
Methyl amino acids
{Lys(Me)}
{Lys(Me2)}
{Lys(Me3)}
N-Methyl amino acid
{N-Me-Ala}
{N-Me-Phe}
{N-Me-Leu}
{N-Me-Ile}
{N-Me-Val}
{N-Me-Met}
{N-Me-Nle}
{N-Me-Nva}
{Sar}
{N-Me-Ser}
{N-Me-Tyr}
{N-Me-Thr}
{N-Me-Asp}
{N-Me-Glu}
N-Me-beta-Ala
N- and C-terminal modifications
Acetyl, formyl, [ac], [for]
Aminooxyacetyl, [aoa]
Azide, [N3]
N-terminal KLH (keyhole limpet hemocyanin) conjugation
Pyroglutamate, [pyr]
S-acetylthioacetate, [sata]
Succinyl, [suc]
C-terminal modification
Amide, [NH2]
Alkylester, [COOMe], [COOEt]
Cysteamide, [cya]
Ethylenediamine, [ed]
Hydroxysucinimide ester, [Osu]
C-terminal KLH (keyhole limpet hemocyanin) conjugation
N-methylamide, [NHMe]
IgG antibodies conjugation: IgG-FITC, IgG-HRP, IgG-Biotin
Internal & terminal Spacer
C6 spacer
Chemical name is: 6-aminohexanoic acid NH2-(CH2)5-COOH
Synonym: AHX.
Cyclische Peptide
Peptide mit 1, 2 oder 3 Disulfid-Brücken.
z.B.
Peptide mit einer Cys-Cys Disulfid-Bücke und einer Länge von 10-12 Aminosäuren [ L-AA] *)
Menge: 2 mg
Reinheit: >95%
Preis: ab 399,05 € **)
Genosphere Biotechnologies Peptidsyntheseservice.
eMail: info@genosphere-biotech.de
*) andere Längen sind ebenfalls erhältlich.
**) zzgl. Versandkosten pro Lieferung.
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