Mammalian cells

Service description

• Codon optimisation and gene synthesis
• Subcloning of gene in CMV promoter-based expression vectors
• Transform construct into E coli strain
• Prepare plasmid and transfect transient cells strains
• Screening for expression
• Scale up culture cells for transfection
• Affinity purification (Ni2+, GST, etc)
• QC: SDS-PAGE analysis, western blot, purity, concentration
• Project report

Lead time: 7-8 weeks

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