Herstellung polyklonaler Antikörper
polyklonale Antikörper
Genosphere Biotechnologies, Paris | Antikörperproduktionsservice | polyklonale Antikörper | Host - Kaninchen | Meerschweinchen | Ratte | Maus | Huhn | Ziege.
Seit 1997 stellt Genosphere Biotechnologies seinen Immunisierservice Kunden weltweit zur Verfügung. Polyklonale Antikörper im Kundenauftrag, generiert in Maus, Ratte, Meerschweinchen, Kaninchen, Ziege und Huhn - Quietly reliable.
Die Antikörper werden in den vier Reinheitsstufen: "crude", "total IgG", "affinity chromatography", "double affinity chromatography" angeboten.
Als Host stehen : mouse, guinea pig, rat, rabbit, chicken, goat zur Auswahl.
See also: Avian IgY antibodies (Hen)
Quietly reliable - Peptidsyntheseservice und Antikörperproduktion aus einer Hand !
Antibodies development services
+ Polyclonal and Monoclonal
+ Anti-peptide, anti-protein
+ Phospho-specific antibody development
+ Hosts: goats, rabbit, guinea pig, rat, mouse, hen
+ Purification: protein A, affinity purified
+ High ELISA titer guarantees
anti-Peptide pAb development
| Host & Purification method | Delivery |
|---|---|
| 2 rabbits; Affinity chromatography purification. anti-Peptide Abs. Bestellnummer: GBAKH270423AFFRB | 2-5 mg specific antibodies. Pre-immune sera. Report with ELISA data > 1: 40.000 |
| 2 rabbits; total IgG chromatography purification. anti-Peptide Abs. Bestellnummer: GBAKH150423TIGGRB | 15-25 mg total IgG antibodies. Pre-immune sera. Report with ELISA data > 1: 30.000. |
|
2 hen ; total IgY chromatography purification. anti-Peptide IgY Abs. Bestellnummer: Bestellnummer: GBHP1AB05-01 |
50-100 mg total IgY purified antibodies derived from yolk (eggs). ELISA, guaranteed titer >1: 30.000 |
Polyclonal chicken antibodies (IgY) for research.
Why avian Chicken affinity purified IgY ?
- Because, higher titres against highly-conserved mammalian gene products !
Easier double immunostaining
Chicken IgYs can be used together with mouse and rabbit antibodies, without the danger of cross-reactivity. Secondary antibodies against chicken IgYs don’t cross-react with mammalian IgGs.
Higher detection signals
Chicken IgYs contain a larger glycosylation index, allowing more labeling with HRP and other antibody tags. This produces higher detection signals.
No "Fc domain" within the stem portion of a chicken IgY
This provides several advantages over rabbit IgGs:
It reduces the likelihood of having false positives in diagnostic applications, since it is the mammalian Fc domain that binds "rheumatoid factors" or other naturally occurring anti-Fc antibodies.
Does not activate mammalian complement systems, allowing the use of chicken IgYs in in vivo applications.
Does not bind to mammalian Fc receptors, avoiding non-specific binding to cells expressing such receptors (e.g., macrophages and dendritic cells).
Affinity purified antibody
examplified ELISA Result:
ABS 405 nm
Antibody
Purification method : affinity column
Rabbit No 1
dilution Pre-immune Purified
1:25 000 0.136 2.00
1:50 000 0.052 1.55
1:100 000 0.035 1.26
Rabbit No 2
dilution Pre-immune Purified
1:25 000 0.147 2.02
1:50 000 0.062 1.98
1:100 000 0.034 1.42
Interpretation of ELISA titer: < 1:1 000 = Below average ; 1:1 000 - 1:10 000 = Average ;
1:10 000 = Good; 1:10 000-100 000 = Excellent
ELISA Procedure:
Antigens are coated on EIA strips at 10μg per well. Wells are washed with 200 μl PBS buffer.
Antisera is diluted in series, added in separate wells , and incubated for 2 hours.
Unbound antibodies are washed and anti-Rabbit IgG-HRP conjugate is added.
Plates are washed and color development run for 15 min. with TMB substrate.
Absorbance is read at 405 nm (2.00 AUFS).
Color intensity is directly proportional to the amount of antibodies.
Antibody is positive if absorbance is >2 folds over that of pre-immune serum.
Background absorbance for pre-immune serum can reach 0.1 to 0.3
Pigeon Tektin-5 (TEKT5)
Multi-Peptide Co-Immunization
In some instances, in order to improve the chances of producing a successful antibodies pool, one may choose several target peptides within the same protein sequences and use them as antigen in a co-immunization protocol. Having several epitopes derived from a single protein will increase the chances of obtaining a pool of antibodies that will recognize the target protein. Some background signal increase may be experienced in some instances.
We will synthesize, purify and conjugate each peptide separately. Individual KLH conjugates are then mixed in equimolar ratios and used as immunogen.
anti-Peptide IgY : ab 956 €
Zugriffe heute: 4 - gesamt: 4009.